谢建台教授：基于激光的敞开式离子源质谱
导读：
7月30日至8月1日，2010年全国质谱会暨第三届世界华人质谱研讨会在吉林省长春市召开，本届大会共有近600位质谱相关人士参加，来自海内外的华人顶级质谱专家、学者在会上大家带来了精彩的学术报告
台湾质谱学会理事长谢建台教授
台湾质谱学会理事长谢建台教授在本届大会上演讲了题为"基于激光的敞开式离子源质谱"（Laser-Based Ambient Ionization Mass Spectrometry）的报告。
在报告中，谢教授主要向大家介绍了电喷雾辅助的激光解吸离子化（ELDI）技术、激光诱导的声解吸（LIAD）技术及两技术在通量的薄层/质谱(TLC/MS)分析、分子成像分析、化学反应监测、蛋白结构研究等方面的应用情况。
ELDI技术和LIAD技术在质谱上应用，无需样品制备，即可在敞开式离子源条件下，分析固体或液体样品，以下是英文内容摘要：
Electrospray-assisted laser desorption ionization (ELDI) and laser-induced acoustic desorption (LIAD) mass spectrometry (MS) are useful techniques for directly characterizing small chemical or large biological components in solid or liquid under ambient conditions. Sample pretreatment is usually unnessary for both techniques. The analytes molecules in the samples are desorbed by the action of a pulsed laser without the assistance of organic matrix. The desorbed molecules are subsequently ionized in electrospray plume generated by electrospraying and acidic methol solution. Recently, we have modified the existing ELDI and LIAD source so unique applications are performed: 1) High throughput TLC/MS analysis - the TLC plate was linearly scanned by the laser from either side of the plate. Typically the systems allow to continuously screening up to 400 TLC plates per day. 2) Molecolar imaging analysis - with the assistance of a stepper motor for precisely moving the sample plate and a high spatial resolution laser, the distribution of the predominant chemical compounds on a particular sample surface were obtained. The molecular images based on volatile and non-volatile chemical compounds on dry fungus and plant slices were obtained. 3) Monitoring chemical reaction - the states of ongoing chemical reactions - nanoparticle catalyzed photodecomposition of dye molecules in different organic solvents, were continuously monitored. 4) Protein structure study - the change of protein structure was studied via H/D and D/H exchange. The amino acids, peptides, and proteins with different exchangeable H atoms were chosen to ascertain the number of H/D or D/H exchanges in a reactive-ELDI/MS system. In addition, a new configuration of the electrospray system was built for both ELDI and LIAD sources. A multiple electrosprayer system was used to generate an atmosphere containing homogenously charged species. The efficiency of post ionization of the desorbed analyte molecules in the new ion sources is greatly increased. Different solvent systems can be simultaneously used to produce electrospray plumes to ionize the analyte molecules with different polarities.
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